Research Information System
Thesis Type: Doctorate
Institution Of The Thesis: Gazi Üniversitesi, Fen Bilimleri Enstitüsü, Turkey
Approval Date: 2015
Student: NAZMİYE ATASEVEN
Supervisor: DENİZ YÜZBAŞIOĞLU
Abstract:Determining genotoxic potentials of food additives which increasingly becomes a part of life in recent years is quite crucial in terms of human health and life quality. In this study, in vitro genotoxic effects of monosodium glutamate (MSG), sodium propionate and potassium propionate on that any study is not carried out before (except sister chromatid exchange assay for MSG) despite their extensive use were examined in human lymphocytes through chromosome aberration (CA), sister chromatid exchange (SCE), micronucleus (MN), comet and random amplified polymorphic DNA-Polimerase chain reaction (RAPD-PCR) tests. To assess genotoxic effects 250; 500; 1000; 2000; 4000; 8000 µg/mL concentrations of MSG, 15,62; 31,25; 62,50; 125,00; 250,00; 500,00 µg/mL concentrations of sodium propionate and 7,81; 15,62; 31,25; 62,50; 125,00; 250,00 µg/mL concentrations of potassium propionate were used. These food additives significantly increased the frequency of chromosome aberrations, sister chromatid exchanges and micronucleus compared with the control. In the comet assay these additives statistically increased mean comet tail intensity, tail length and tail moment compared with control. In this thesis, in vitro genotoxic effects of the MSG, sodium propionate and potassium propionate were also investigated on human peripheral lymphocytes by analysing the RAPD-PCR with arbitrary 10-mer primers. The changes occurring in RAPD profiles following MSG and potassium propionate treatments include increase or decrease in band intensity and gain or loss of bands. However, sodium propionate treatment induced any changes with primers we used. The obtained results showed that these three food additives have genotoxic effects on human lymphocytes in vitro.