MERKEZİ KOMPOZİT TASARIM KULLANARAK Cr(III)'ün MAYA BAZLI BİOSORBENT İLE ZENGİNLEŞTİRİLMESİ VE TAYİNİ

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Thesis Type: Postgraduate

Institution Of The Thesis: Gazi University, Fen Bilimleri Enstitüsü, KİMYA ANABİLİM DALI, Turkey

Approval Date: 2024

Thesis Language: Turkish

Student: ALIYA AMANZHOL

Principal Supervisor (For Co-Supervisor Theses): Özcan Yalçınkaya

Co-Supervisor: Berat Çınar Acar

Open Archive Collection: AVESIS Open Access Collection

Abstract:

In this study, the preconcentration of Cr(III) ion in microorganism immobilized on perlite adsorbent by solid phase extraction was investigated. As the microorganism was tested Pichia kudriavzevii JD2. Cr(III) determination was made by flame atomic absorption spectrometry. The effects of pH, amount of adsorbent, volume and type of recovery solution, and flow rate and volume of the sample solution on the recovery efficiency of Cr(III) ion were investigated. By applying the column technique, the optimum preconcentration conditions were determined as pH 4, recovery solution 2 mol/L HNO3 and volume 10 mL, and sample flow rate 1-3 mL/min. Under the optimum conditions, the recovery efficiency of Cr(III) ion was found to be 100,1±0.3% of perlite immobilized on Pichia kudriavzevii JD2 with a 95% confidence level. Analytical variables with limit of detection (LOD) of 4.8 µg/L and limit of quantification (LOQ) of 15.8 µg/L were determined for the Cr(III) ion. The accuracy of the method was determined using standard reference materials (CRM). The relative error of the recovery efficiency was determined to be less than 10%. The method was applied to the determination of Cr(III) in various water samples such as tap water and mineral waters.