Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi Üniversitesi, Fen Bilimleri Enstitüsü, Turkey
Approval Date: 2014
Student: SEDA KIRAÇ
Supervisor: NESLİHAN GÜNDOĞAN BAKIRCI
Open Archive Collection: AVESIS Open Access Collection
Abstract:In this study, 52 fish samples, which are provided for consumption in Ankara, were used as material. Gr(-) bacteria have been isolated and identified from samples. The research, to determine the extended spectrum beta-laktamase activities (ESBL) and the antibiotic resistances of the Gr(-) bacteria isolates, was conducted by using double disk synergy and Kirby-Bauer disk diffusion method respectively. Also proteolytic, lipolytic enzyme activities of Gr(-) bacteria isolates were determined and slime, hemolysis and siderophore tests were applied. The distribution rates among 221 Gr(-) bacteria strains isolated from fishes were %21.3 Enterobacter spp., %14.5 Escherichia spp., %11.3 Citrobacter spp., %9.5 Klebsiella spp., %9.5 Hafnia spp., %9.5 Pseudomonas spp., %6.3 Serratia spp., %3.6 Proteus spp., %3.6 Pantoea spp., %2.7 Acinetobacter spp., %2.7 Aeromonas spp., %2.3 Providencia spp., %1.8 Rahnella spp., %0.5 Morganella spp., %0.5 Leclercia spp., and %0.5 Ewingella spp. From a total of 221 Gr(-) bacteria 121 (54.8%), 51 (23.1%), 20 (9%) isolates were positive for slime, hemolyse and siderofor test recpectively. From 221 Gr(-) bacteria isolates 8 of the isolates (%3.6) strong positive, 13 of isolates (%5.9) medium positive, 13 of the isolates (%5.9) weak positive have been identified proteolytic activity at 4°C. 24 of the isolates (%10.9) strong positive ,11 of isolates (%4,9) medium positive, 1 of the isolates (%0.5) weak positive have been identified proteolytic activity at 20°C. 12 of the isolates (%5.4) strong positive, 11 of isolates (%4,9) medium positive, 18 of the isolates (%8,1) weak positive have been identified proteolytic activity at 3°C. From 221 Gr(-) bacteria isolates, 12 of the isolates (%5.4) strong positive ,15 of isolate (%6.8) medium positive, 13 of the isolates (%5.9) weak positive have been identified lipolytic activity at 4°C. 24 of the isolates (%10,9) strong positive, 13 of isolates (%5.9) medium positive, 20 of the isolates (%9) weak positive have been identified lipolytic activity at 20°C. 5 of the isolates (%2.3) strong positive, 10 of isolates (%4,5) medium positive, 24 of the isolates (%10,9) weak positive have been identified lipolytic activity at 20°C. The highest resistance rates of Gr(-) bacteria strains were aganist amoxicillin (85.9%), amoxicillin - clavulanic acid (66.1%), ampicillin (63.3%) and ampicillin-sulbactam (47.9%). 60 (27.1%) of 221 identified Gr(-)bacteria isolates were displayed extended spectrum beta lactamase (ESBL) positive. The distribution rates of 60 ESBL (+) Gr(-) bacteria strains were 28.3% E. coli, 11,7% K. oxytoca, 10% C. freundii, 6.7% A. hydrophila, 6.7% C. amalonaticus, 6.7% H. alvei, 6.7% S. Fonticola, 5% P. vulgaris, 3.3% A, iwoffii, 3.3% E. cloacae, 3.3% P. agglomerans, 1.7% E. sakazakii, 1,7% E. vulneris, 1,7% K. pneumoniae, 1,7% P. aeruginosa, 1,7% S. marcescens. The highest resistants rates of ESBL (+) Gr(-) bacteria strains were determined aganist amoxicillin (86.7%), amoxicillin clavulanic acid (65%), ampicillin (60%) and ampicillin - sulbactam (36.7%).