Research Information System
Thesis Type: Expertise In Medicine
Institution Of The Thesis: Gazi Üniversitesi, Tıp Fakültesi, Turkey
Approval Date: 2011
Student: ŞEVKİ MUSTAFA DEMİRÖZ
Supervisor: ABDULLAH İRFAN TAŞTEPE
Abstract:Lung cancer is the most common cause of death due to cancer in both men and women throughout the world. The epigenetic alterations include DNA methylation, and histone modification. Alterations in epigenetic pathways have been shown to be implicated in common human diseases. Histone modifications are controlled by histone modifying enzymes. Aim of this study is to identify the relationship between mRNA expression of histone modifying genes and development of NSCLC. In this study, the expression profiles of three histone modifying genes of cancer tissue and noncancerous part from 44 patients of non small cell lung cancer were investigated. Total cellular RNA was isolated from tissue samples. The mRNA expression levels of SETD2, JMJD2C and TIP60 genes were measured by quantitative real-time polymerase chain reaction (RT-PCR). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was used as the housekeeping gene in order to normalize expression levels. When the mRNA expression levels of these genes in lung cancer tissues were compared with those of normal lung tissues, mRNA expression level of JMJD2C did not differ significantly (p>0.05). However, there were a significant decrease in mRNA levels of SETD2 and TIP60 genes in malign tissues (p>0.05). Our findings indicate a possible association of SETD2 and TIP60 genes mRNA expression with the pathogenesis of NSCLC. To confirm these findings, further molecular investigation is needed.