Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi University, Fen Bilimleri Enstitüsü, Turkey
Approval Date: 2024
Thesis Language: Turkish
Student: İbrahim Wazir ANWAR
Supervisor: Yusuf Kalender
Open Archive Collection: AVESIS Open Access Collection
Abstract:
With the development of nanotechnological applications, it has been observed that a wide variety of nanoparticles have begun to be produced and scientific research in this field has become widespread. Due to the specific properties of designed nanoparticles, the frequent use of nanoparticles in the automotive, biomedical and pharmaceutical industries has led to the spread of many nano-application-based products into the environment and, as a result, they have become products that directly or indirectly threaten the environment and public health. Nickel oxide microparticles (NiO-MP) and nickel oxide nanoparticles (NiO-NP) are widely used in industry and have been reported to induce oxidative stress, interact with cell membranes and proteins, and accumulate in tissues and organs. This study aimed to investigate the effects of NiO-MP and NiO NPs on rat brain tissue. In the study, 42 male Wistar rats were divided into 7 groups. 1. Control group, 2. NiO-MP (150 mg/kg/day) orally administered group, 3. NiO-MP (20 mg/kg/day) intraperitoneally administered group, 4. NiO-MP (1 mg/kg) /day) intravenously administered group, 5. NiO-NP (150 mg/kg/day) orally administered group, 6. NiO-NP (20 mg/kg/day) intraperitoneally administered group, 7. NiO-NP (1 mg /kg/day) intravenously administered group. For this study, G.Ü. permission was obtained from the Animal Experiments Local Ethics Committee (G.Ü. ET-21.033). After 21 days, malondialdehyde (MDA), the end product of lipid peroxidation, and antioxidant enzyme activities [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST)] were measured by spectrophotometer in the brain tissue of rats. As a result of the study, a statistically significant increase was observed in terms of MDA when the control group and the NiO-MP and NiO-NP applied groups were compared. When the NiO-MP and NiO-NP applied groups were compared with the control group, a statistically significant decrease was observed in antioxidant enzyme activities (SOD, CAT, GPx, GST). As a result, NiO-MP and NiO-NP applied groups caused an increase in the MDA levels and a decrease in antioxidant enzyme activities in the rat brain tissues. In the histopathological examinations performed on the brain tissue of rats, pathological findings were observed in the nerve cells of the intravenously administered groups.
Key Words : Nanoparticle, Nickel oxide, Antioxidant, Oxidative Stress