Research Information System
Thesis Type: Doctorate
Institution Of The Thesis: Gazi University, Sağlık Bilimleri Enstitüsü, Turkey
Approval Date: 2008
Thesis Language: Turkish
Student: Evrim TÜZÜNER
Supervisor: ALEV ALAÇAM
Abstract:This study aimed to investigate the fluoride concentrations in saliva and urine after drinking F-milk compared to F-water and F-free milk. 28 children aged 3-6 years were subjected to four different 7-day experimental regimens: Group 1. F-milk with breakfast, Group 2. F-milk before breakfast, Group 3. F-water before breakfast, Group 4. F-free milk before breakfast. F- concentrations in whole saliva and urine was determined according to background, baseline, and 7th day evaluations using micro-diffusion method. There were no statistically significant differences between the baseline values in all groups (p>0,05). However, the salivary and urinary F- concentrations were significantly increased with consuming fluoridated milk and water compared to F-free milk (p<0,05). Drinking F-milk with breakfast or before breakfast did not influence the salivary and urine Flevels significantly (p>0,05). The effect of fluoridated milk on enamel structural was evaluated in vitro by 3 depth F analysis method, microhardness tests and Lazer fluoresans measurements. In this experimental pH cycling study 60 extracted molar teeth were exposed to demineralisation solution for 4 h immediately followed by another 4-h period in either F-milk or F-free milk. During the rest of the day and over the weekends the samples were stored in the remineralisation solution. All samples were examined at baseline, 2nd and 4th weeks. After 4 weeks enamel F- contents of the fluoridated milk samples were found higher in the first depth compared to the other groups (p<0,05). Also enamel F- content was decreased in deeper layers compared to surface layers in all groups. The microhardness values decreased with time in all groups, but the decrease was less in test group after 2 and 4 weeks (p<0,05). Lazer fluoresans values increased with time in both groups but the increase was more marked in the control group after 4 weeks (p<0,05). In vivo and in vitro results showed that fluoridated milk significantly increased the salivary and urinary concentrations and the structural enamel F- levels. Also, it was found that fluoridated milk increased the microhardness values of enamel in a low pH cycling model showing a decrease in enamel demineralisation. It can be concluded that as a cheap and safe alternative for caries preventive measure, fluoridated milk programmes should be encouraged.