Research Information System
Thesis Type: Expertise In Medicine
Institution Of The Thesis: Gazi Üniversitesi, Tıp Fakültesi, Turkey
Approval Date: 2017
Student: İHSAN ÖZDEMİR
Supervisor: FARUK GÜÇLÜ PINARLI
Abstract:Purpose: The aim of the study is to investigate the expression and methylation status of 7 distinctive genes with tumor suppressing properties in childhood lymphomas. Patients and Methodology: A total of 96 patients (41 HL, 15 NHL and 40 non-malignant lymph node proliferation) who are followed up between 2000-2015 in the Pediatric Oncology Division of Pediatry Department of Gazi University and whose pathological examination were performed in our center are included in the research. Specific PCR studies of RT-qPCR and methylation were performed by making DNA and RNA isolation in tissue samples. Results: When patient groups and control groups are compared, we found that SPI1, PRDX2, KLF4, DLEC1 DAPK1 genes are significantly less expressed with respect to control group. However, expression of CDKNA2 and FOXO1 genes in the patient and control groups were not statistically different. The methylation ratios of all genes excluding the CDKN2A and FOXO1 genes in HL and NHL groups were significantly higher than the controls. Conclusion: We showed that SPI1, PRDX2, DLEC1, KLF4, DAPK1 genes are silenced with hypermethylation in the tumor tissue samples of the childhood HL and NHL patients. Due to the fact that CDKN2A gene is not expressed in both of the patient and control groups, we concluded that it is not specific to malignancy. As FOXO1 gene were expressed in both of the patient and control groups, its relation with malignity could neither be found.