Research Information System
Thesis Type: Expertise In Medicine
Institution Of The Thesis: Gazi Üniversitesi, Tıp Fakültesi, Turkey
Approval Date: 2013
Student: ZÜBEYDE LALE
Supervisor: IŞIL FİDAN
Abstract:HBV is a DNA virüs, genus of orthohepadnavirus at Hepadnaviridae family,hepatotrophic, enveloped and partially double-stranded. Infectious agent suse a variety of mechanisms to initiate or sustain otoimmunity. Of these mechanisms, several mechanisms may be seen such as molecular affinity, and releasing of antigen-kept secret when host tissue and cells are damaged, changing of host cell proteins, and activation of autoreactive T lymphocyte. Several infectious agents like viruses, bacteria and parasites can trigger so many different mechanisms by Autoimmunity. Molecular mimicry, cryptogenic antigens as a result of the expression of host tissue destruction, transformation of host cell proteins and an activation are some of autoreactive mechanisms. HBV infection may trigger autoimmunity as several infections, and also may cause many changes mediators in the immune system. Therefore, in our study, we aimed to detect the formation of autoantibodies and changes in serum cytokine levels in patients with chronic hepatitis B. Materials and Methods: In this study, 50 adult patients with HbsAg, and HBV DNA-positive and chronic hepatitis B, HBsAg (+) ,over the age of 18, were selected and 25 patients with HBV DNA (- ) , inactive HBsAg carrier, and HBsAg (-) were selected from healthy control group as samples. HBV DNA was determined by quantitative real-time polymerase chain reaction. ANA, ASMA, AMA, LKM autoantibodies were examined by using indirect fluorescent Ab method. The definition of autoantibodies were made by using immunoblotting method from samples found ANA positive by using IFA method. RF and anti-CCP, antiphospholipid G, antiphospholipid M, anticardiolipin autoantibodies were examined by the ELISA method. Also,Serum cytokin elevels were examined by ELISA. Results: The fact that at least one positive of ANA, AMA, ASMA and LKM autoantibodies were found to be statistically significant between inactive HBsAg carrier and control groups in the samples of patients with chronic hepatitis B. ANA 44%, AMA 10%, ASMA 16%, LKM 2% positivities were found in samples of patients with chronic hepatitis B.While ANA 28 %, ASMA 20 % positivities were detected in serum samples of inactive HBsAg carriers, ASMA, AMA and LKM antibody positivity could not be detected. There was no statistically difference between ANA positive and ANA negative groups in terms of HBV DNA viral load. In all three groups, RF positivity was found 30 %, 24 %, 8 %, respectively.Anti-CCP was found ( 4%) in examples of chronic hepatitis B group. In our study, 4% antiphospholipid G positivity and 12% antiphospholipid M positivity were found in chronic hepatitis B group. Anticardiolipin antibodies were found in 14% of cases of chronic hepatitis B group. Serum IL-17, IL-22, IL-23 levels were found to be significantly increased when compared the examples of chronic hepatitis B to inactive HBsAg carrier group and the control group Conclusion : To examine autoantibodies and cytokine response may help to discriminate autoantibody response induced by HBV infection, and contribute to find new treatment methods. However, comprehensive studies are needed to get a beter understanding of autoimmune mechanisms caused by HBV, HBV infection, and host immune reaction.