Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi Üniversitesi, Fen Bilimleri Enstitüsü, Turkey
Approval Date: 2014
Student: KÜBRA SEVİMLİ
Supervisor: DENİZ YÜZBAŞIOĞLU
Abstract:In this study, potential antigenotoxic effects of widely used vitamins α-tocopherol (Vitamin E) and retinol (Vitamin A) were investigated against Mitomycin-C (MMC), an antitumoral agent. Four different assays were performed; Chromosomal Aberration (CA), Sister Chromatid Exchange (SCE), Micronucleus (MN) and Comet. 25, 50, 100 and 200 µg/ml concentrations of α-tocopherol and 005; 0,010; 0,015 and 0,020 µg/ ml concentrations of retinol were used. Different doses of α-tocopherol and retinol were used in combination with 20 µg/ml Mitomycin-C. Three treatment procedures based on 24 and 48 hour treatments were used; pre-, simultaneous and post-treatment. While α-tocopherol reduced CA frequency induced by MMC at pre- and post-treatment, retinol revealed similar effects at three treatment procedures. α-tocopherol and retinol reduced SCE frequency. While α-tocopherol reduced MN frequency especially at simultaneous treatment, retinol decreased MN frequency at all 24 hour based procedures. In Comet assay, α-tocopherol did not show protective effect on primer DNA damage. However, retinol reduced DNA damage at only simultaneous treatment procedure. In general, none of the antioxidants could regulated MMC induced mitotic inhibition. It is thought from obtained results that both of α-tocopherol and retinol has protective and healing effects against MMC induced genotoxicity in vitro human lymphocytes.