Research Information System
Thesis Type: Doctorate
Institution Of The Thesis: Gazi University, Turkey
Approval Date: 2017
Thesis Language: Turkish
Student: Saadet Özen Akarca Dizakar
Supervisor: SUNA ÖMEROĞLU
Abstract:Nowadays, it is notable that the increase in the incidence of metabolic syndrome (MetS) and related clinical cases correlated with the increased of fructose rich food consumption and environmental and oral exposure to chemical substances known as endocrine disruptors. Fructose feed has recently increased and higher fructose consumption has associated with the increased of diseases such as dyslipidemia, insulin resistance, hypertension and hyperuricemia. BPA exhibits estrogen-like activity that cause toxicity in reproductive organs suppresses spermatogenesis, decreases sperm production and fertility rates. According to information given above, we used 42 adults, male Sprague Dawley rats that were divided into 7 groups. The groups were arranged as follows, Group 1: (n=6) Control, Group 2: (n=6) Rats were treated with fructose, Group 3: (n=6) Rats were treated with BPA, Group 4: (n=6) Rats were treated with fructose and BPA contemporaneously, Group 5: (n=6) Rats were treated with fructose and Melatonin, Group 6: (n=6) Rats were treated with BPA and Melatonin, Group 7: (n=6) Rats were treated with fructose and BPA and melatonin. At the end of the experiment, epididymal tissue was removed and analyzed by using histochemical and immunohistochemical procedure. Sperm samples were counted and sperm motility and viability were investigated. Furthermore, we examined the effects of strong antioxidant melatonin on this experimental process. We found that administration of BPA, fructose, and concurrently both of these causes damaged to epididymal tissue and sperm samples on the other hand melatonin was partially successful in preventing this damage.