Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi University, Turkey
Approval Date: 2015
Thesis Language: Turkish
Student: Damla DERNEK
Supervisor: SUNA ÖMEROĞLU
Abstract:Bisphenol A (BPA) is one of the widely used endocrine disrupting chemicals in the world. Studies on female rats show that BPA effects on the period of puberty, ovarian and uterine weights, anogenital distance, the vaginal opening time and estrous cycle. Melatonin is a hormone which is synthesized and secreted from mammalian pineal gland, ovary, lens, bone marrow cells, the gastrointestinal tract and bile. Some studies which are situated in literature that melatonin reduces the toxicity of some drugs, induces follicular development, and increases the quality of oocyte, due to its antioxidant property. In this study, compatible with the literature, Female rats exposed with BPA in neonatal period. After this exposure, there was detected degenerations in uterus tissue of these rats. Possible effects of melotonin investigated against these degenerations implement in prepubertal period. For these purpose, twenty-four female Wistar-albino rats were divided equally into four groups. Rats in Group I were used as control (sesame oil + ethanol). Rats in Group II were injected daily with BPA (100 mg/kg) subcutaneously for 10 days (PND0-PND10). Rats in Group III were injected daily with melatonin (10 mg/kg) subcutaneously for 10 days (PND20-PND30). Lastly, rats in Group IV were injected daily with BPA (100 mg/kg) (PND0-PND10) and melatonin (10 mg/kg) (PND20-PND30) subcutaneously for 10 days. At the end of the experimental period, all rats were sacrificed on the PND70 stage and the uterine tissue of rats were removed. As a result of studies, it was observed that BPA decreases the time of the vaginal opening, increases apoptotic reaction rates of the uterus luminal and glandular epithelium, causes early onset puberty period and degenerative changes of the uterine tissue in rats. BPA was also increased the rats body/uterus weight but the results were not statistically significant (These results coherent with results of BPA uterine luminal epithelium height, myometrium thickness and uterine glands.). On the contrary, BPA increased uterine lumen diameter and the results were statistically significant. In rat groups which exposed with BPA+Melatonin, the degenerative changes also changed positively. Furthermore, rat groups which exposed with melatonin findings were same with Control Groups.