Research Information System
Thesis Type: Doctorate
Institution Of The Thesis: Gazi University, Turkey
Approval Date: 2017
Thesis Language: Turkish
Student: Miyaser Demirel
Supervisor: NURSEL DİLSİZ
Abstract:In this study, samples of sponge, powder and film containing hemostatic agents were prepared in order to develop biomedical material containing hemostatic properties. For this purpose, chitosan was used as the basic hemostatic agent. Freeze drying (lyophilization) and grinding methods were used as the production technique. Porosity, water absorption rate, permeability, hemostatic activity, blood absorption, activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and fibrinogen (FIB) tests were first applied to characterize the hemostatic samples. As a result of these tests, samples having the best hemostatic properties were analyzed for hemolysis, platelet aggregation, antibacterial activity. The scanning electron microscopy (SEM), particle size distribution, fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) tests were also applied. As a result of the tests applied to the sponge samples, as the lyophilization time increased and the amount of lyophilized sample decreased, porosity and pore volume values increased, density, water absorption rate and blood absorption rate decreased. The platelet aggregation and hemolysis test were performed to the sample showing the best blood absorption behavior within the sponge samples. The results showed that this sample did not cause platelet aggregation and was hemolytic. The sample showing the best blood absorption behavior of the powder samples obtained platelet aggregation and was hemolytic. The results of APTT, PT, FIB and TT tests applied to sponge, powder and film samples showed that APTT, PT and TT values of all samples were lower than negative control and FIB values were higher than negative control. It was determined that all samples accelerated the blood clotting mechanism.