Research Information System
Thesis Type: Doctorate
Institution Of The Thesis: Gazi Üniversitesi, Fen Bilimleri Enstitüsü, Turkey
Approval Date: 2014
Student: SEVCAN MAMUR
Supervisor: DENİZ YÜZBAŞIOĞLU
Abstract:Today, the incidence of chronic kidney failure (CKF) is a disease that is increasing rapidly. The incidence of cancer in chronic kidney disease found to be higher when compared to the general population. The mechanism of this situation has not been totally known yet. The role played by dialysis in genome damage is a controversial issue. In addition, diabetes is an important cause that leads to chronic kidney failure in chronic kidney disease. According to the literature researches, no studies have been conducted to investigate genome damage in chronic kidney failure patients with diabetes in human lymphocytes. For this reason, we aimed to determine genetic damage in chronic kidney disease in hemodialysis patients with and without diabetes by using chromosomal aberrations (CAs), sister chromatid exchanges (SCEs), micronucleus (MN) and Comet tests. According to this study, in CKF patients the frequency of chromosome aberrations, sister chromatid exchanges and micronuclei statistically increased compared to controls. In addition, comet tail length and tail moment were significantly higher in chronic kidney failure patients than the control group. But, there was no difference between diabetic and non-diabetic CKF patients in terms of genotoxicity test results. In CKF patients, mitotic (MI), replication (RI) and nuclear division indices (NDI) were significantly decreased compared to controls. In addition, replication (RI) and vii nuclear division indices (NDI) were significantly decreased in diabetic CKF patients than the non-diabetic CKF patients. Mitotic index significantly decreased in CKF patients with ferritin levels higher than 500 ng/ml. In CKF patients with ageing levels less than 50 was statistically increased the frequency of sister chromatid exchanges compared to ageing levels higher than 50. Nuclear division index was significantly decreased in CKF patients with ageing levels higher than 50. The comet tail intensity and tail moment in CKF patients with body mass index (BMI) levels higher than 25 (kg/m2) significantly increased compared the body mass index (BMI) levels less than 25 (kg/m2). Morever, the comet tail length in CKF patients with parathyroid hormone (PTH) levels higher than 300 pg/ml significantly increased compared to the parathyroid hormone (PTH) levels less than 300 pg/ml. There was a positive and significant correlation between the Comet tail length and PTH. There was no relation between the CA, SCE, MN, DNA damage and duration of hemodialysis treatment. The results of experiments showed that clastogenic and mutagenic effects were increased in patients with chronic kidney failure undergoing hemodialysis compared to the controls and diabetes mellitus also affected the cell proliferation.