Determination of ephedrine, norephedrine and guaifenesin in syrup preparations by capillary electrophoresis

Thesis Type: Doctorate

Institution Of The Thesis: Gazi Üniversitesi, Sağlık Bilimleri Enstitüsü, Turkey

Approval Date: 2014




The aim of the study was to develop and compare two capillary electrophoresis methods for the determination of Ephedrine, Norephedrine, Guaifenesin and Methylparaben. In MEEKC and MEKC methods, parameters were optimized according to the best separation and determination results. In MEKC method, the best results were obtained by using a pH 9.5, 5 mM borate buffer electrolyte solution including 70 mM SDS and 15% ACN. While optimizing the capillary conditions, the best separation was obtained by using 30˚C cassette temperature and +22 kV separation voltage with an injection time of 20 s. In MEEKC, the optimum conditions were obtained by using a mixture of pH 11, 10 mM borate buffer 96.7% (w/w) and 1.4% SDS , 1.2% 1-butanol and 0.7% ethyl acetate in microemulsion solution. Injection time of 20 s and temperature of 40˚C were chosen for the separation. The linearity of the methods were between 1-25 µg.mL-1 for both MEKC and MEEKC. In validation studies, the LOD and LOQ values for GUA, MP, NE and EPH were found to be 0.56; 0.72; 0.38 and 0.25 µg.mL 1 and 0.45; 0.61; 0.38 and 0.46 µg mL 1 respectively by MEKC. In MEEKC LOD and LOQ values for the analytes were found to be 1.68; 2.17; 1.13; 0.76 µg.mL 1 and 1.36; 1.86; 1.15 and 1.38 µg.mL-1 respectively. Finally, the recovery studies were obtained by the analysis of the syrup preparation and the results were analyzed statistically.