Research Information System
Thesis Type: Expertise In Medicine
Institution Of The Thesis: Gazi Üniversitesi, Tıp Fakültesi, Turkey
Approval Date: 2015
Student: ERKUT BAHA BULDUK
Supervisor: GÖKHAN KURT
Abstract:The purpose of this study is the investigation of the effect of Minocycline and the possible correlation between hippocampal microglial activation and S100B serum in an experimental status epilepticus model. In the experiments, 12 week old male Sprague-Dawley rats weighting 200 to 250 grams were used (n=24). Rats were separated in to four groups. Animals induced with lithium and pilocarpin was used in the experiment group. Lithium chloride was given to the experimental animals 24 hours before the injection of pilocarpin (127 mg/kg. i.p.). One dose of Pilocarpin was applied (30 mg/kg, i.p.). Besides that methilschoplamine-bromide (1mg/kg) was given to the animals 30 minutes before pilocarpin injection to remove the cholinergic side effects. Seizures were degreed due to Racine scale. The beginning of SE was defined as the start of lasting seizure activity (without normalizing between seizures). SE time was taken under control using diazepam (30 mg/kg, i.p.) in the rats having SE. Experiment groups are planned as follows: Group 1 (Control,n=6): There was no proceeding on the animals in this group. Group 2 (Sham,n=6): Saline was given to the animals instead of Lithium, methilescopolamine. One dose of diazepam was injected after 2 hours of Saline injection. Group 3 (SE): Lithium Chloride (127 mg/kg. i.p.). was applied to the animals 24 hours before one dose of pilocarpin injection(30 mg/kg, i.p.). Methilescopolamine was also given. Diazepam (10 mg/kg i.p. ) was applied after 60 minutes of pilocarpin injection to end the seizures. At the days of 3, 4, 5, 6 and 7, subcutaneous injection with 5 ml saline and gavage and 1 ml TPN solution is given to the rats two times a day between 09:30-10:30 and 16:30- 17:30 to reduce the death rate. Group 4 (SE + minocycline): the experiment protocol in the group 3 was repeated with the existence of minocycline. Minocycline dissolved in 0.1 M phosphate buffer (pH:7.4, i.p ) was applied with a 50 mg/kg dose at the days 3, 4, 5, 6 ve 7. After Lithium application at eight day all the hippocampuses of the rats was removed and OX-42 and GFAP expression in the dorsal hippocampus was evaluated with immunohistochemical methods. Besides, determination of S100B was performed by taking blood from peripheral veins before the removel of hippocampuses. As a result of this, the high levels of S100B at SE and SE+ minocycline groups with respect to the control group suggest that S100B released from glias as a result of excitotoxic injury is the projection of the ongoing injury and glial reactivity developing against it referring to the literature. Also the significant low level of OX42 (+) microglia number and GFAP (+) astrocyte number in the control group at CA1 with respect to SE group suggests the presence of microglial activation triggered by neuroinflammatory processes in neurodegenerative diseases like epilepsy.