Histopathological and biochemical comparison of Ankaferd Blood Stopper and Low Level Laser Therapy on nerve healing

Thesis Type: Doctorate

Institution Of The Thesis: Gazi Üniversitesi, Sağlık Bilimleri Enstitüsü, Turkey

Approval Date: 2013




Nerve injuries in oral and maxillofacial region lead to loss of sensation, temporary or persistant unless interfere for regeneration. It s been used various medical agents or microsurgery techniques, but recently more conservative treatment modalities were investigated and received successful outcomes with Low Level Laser Therapy (LLLT). The aim of this study is compare the effects of Ankaferd Blood Stopper with Low Level Laser Therapy on nerve healing. 72 Wistar divided 4 main groups; Control, ABS, LLLT, ABS+LLLT and 3., 5, and 12 weeks of sacrification times. The right sciatic nevres are crushed with forceps for 60 seconds and evaluated histopathologically and GAP-43 protein expression by Western blotting biochemically. Regarding the biochemical analyses, GAP-43 expression in all the other groups was significantly higher than control group. For ABS group, GAP-43 was observed significantly higher to be than LLLT group and similar to ABS+LLLT group. The histopathologic examination revealed that ABS caused to temporary depression on edema but increased the capillaries. But later, severe inflamation, axonal degeneration, allergic reaction and fibrotic changes was found in ABS and ABS+LLLT groups. However in LLLT progressively decreasing edema, regeneration and back to normal axonal histological structure. The present study showed that ABS is not a biocampatible material for nervous tissue, lead to fibrotic changes and dejenerations. LLLT is effective in nerve rejeneration. LLLT can be used safely for nerve injury repair, but not ABS. In cases where the use of ABS is necessary, the nerve should be protected from direct contact with ABS as well.