Investigation of sulfonamide antibiotic residues and microbiological contamination level in some animal origin foods consumed in Ankara province


Thesis Type: Postgraduate

Institution Of The Thesis: Gazi University, Sağlık Bilimleri Enstitüsü, Turkey

Approval Date: 2024

Thesis Language: Turkish

Student: Hilal TÜRKAN SERTKAYA

Supervisor: Burak Demirhan

Open Archive Collection: AVESIS Open Access Collection

Abstract:

Consumption of animal foods containing sulfonamide residues poses potential human health risks, including hypersensitivity or anaphylactic shock. Antibiotic residues in chicken are important for human health due to their harmful effects on consumer health. In the study, a total of 84 animal food samples, including 30 chicken meat (A, B and C), 22 chicken eggs (D and E), 22 pasteurized milk (F and G) and 10 raw milk (H) obtained from the Ankara local markets, were analyzed in terms of sulfonamide antibiotic residue, total coliform bacteria and Staphylococcus-Micrococcus spp. The competitive ELISA method was used to detect sulfonamide residues. The classical culture method was used for total coliform bacteria and Staphylococcus-Micrococcus spp. analysis. While sulfonamide residue could not be detected in raw milk, it was detected in pasteurized milk in two samples from two different firms. The mean level of sulfonamide residue in chicken meat and eggs was 2.08±0.13 µg/kg. The mean values of total coliform bacteria, Staphylococcus spp. and Micrococcus spp. in samples were determined as 4.09±0.09 log cfu/g, 2.69±0.18 log cfu/g and 3.18±0.12 log cfu/g, respectively. The total coliform bacteria, Staphylococcus spp. and Micrococcus spp. counts in egg and pasteurized milk samples was less than 102 cfu/g. As a result, misuse of veterinary drugs, excessive use or non compliance withdrawal periods before slaughter may leave residues in animal origin foods that pose a risk to human health. Therefore, regularly monitoring antibiotics in foods of animal origin is very important to protect public health and ensure food safety.

Key Words : Animal origin food, Antibiotic residue, Sulfonamide, Microbiological quality