Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi Üniversitesi, Fen Bilimleri Enstitüsü, Turkey
Approval Date: 2015
Student: GOLCHIN MEMARI
Supervisor: NESLİHAN GÜNDOĞAN BAKIRCI
Open Archive Collection: AVESIS Open Access Collection
Abstract:In this study, 95 fish samples (40 freshwater, 55 seawater) which are provided for consumption in Ankara, were used as material Staphylococcus species were isolated from fish samples and identified. Identified Staphylococcus species were analysed for proteolytic and lipolytic activity at 4°C, 20°C and 37°C, some virulence properties as slime and biofilm formation, DNase and hemolysis activity, and antibiotic resistance with Kirby-Bauerdisk diffusion method were researched. The distribution rates among 217 Staphylococcus isolated from fishes were 38.2% S.warneri, 18.3% S.haemolyticus, 8.3% S.simulans, 6.9% S.intermedius, 6% S.saprophyticus, 4.6% S.aureus, 4.6% S.capitis, 4.1% S.xylosus, 3.2% S.caprae, 1.8% S.epidermidis, 1.8% S.schleiferi subsp.schleiferi, 0.9% S.cohnii subsp.cohnii, 0.9% S.cohnii subsp. urealyticum. In our research, 175 (80.6%) slime (+), 149 (68.7%) DNase (+) and 139 (64.1%) hemolysis (+) are in 217 Staphylococcus isolated from the fish samples. By using microplate method all Staphylococcus isolates after 24 hours of incubation on Tryptone Soy Broth (TSB) 11.1% strong biofilm, 48.8% medium positive, 37.8% weak positive and 2.8% negative biofilm formation have been identified. On the same isolates 2% glucose containing the TSB after 24 hours of incubation in 19.8% strong biofilm, 59.4% medium positive, 16.6% weak positive and 4.6% negative biofilm formation have been identified. In this study, the 6% of identified Staphylococcus strains are resistant to gentamycin, 36.4% resistant to erythromycin, 34.6% resistant to tetracycline, 13.4% resistant to clindamycin, 26.7% resistant to meticillin, 6.5% resistant to amikacin and 3.2% resistant to chloramphenicol. Among studied stain there is no strain results resistant to vancomycin. From 217 Staphylococcus isolates 3 (1.4%) of the isolates strong positive, 10 (4.6%) medium positive, 150 (23%) weak positive and 154 (71%) negative have been identified proteolytic activity at 37°C. We have been identified proteolitic activity at 20°C, 12 (5.5%) of the isolates strong positive, 30 (13.8%) medium positive, 41 (18.9%) weak positive and 134 (61.7%) of isolates negative. 3 (1.4%) of the isolates strong positive, 9 (4.1%) weak positive and 205 (94.5%) negative have been identified proteolytic activity at 4°C. From 217 Staphylococcus isolates, 112 (51.6%) of the isolates strong positive, 46 (21.2%) medium positive, 37 (17.1%) weak positive and 22 (10.1%) negative have been identified lipolytic activity at 37°C. We have been identified lipolytic activity at 20°C, 137 (63.1%) of the isolates strong positive, 32 (14.7%) medium positive, 28 (13%) weak positive and 20 (9.2%) negative. 94 (43.3%) of the isolates strong positive, 52 (24%) medium positive and 40 (18.4%) weak positive and 31 (14.3%) negative have been identified lipolytic activity at 4°C.