Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi University, Sağlık Bilimleri Enstitüsü, FARMAKOGNOZİ ANABİLİM DALI, Turkey
Approval Date: 2019
Thesis Language: Turkish
Student: MUSTAFA SAK
Supervisor: Esra Akkol
Abstract:
Hyoscyamus niger L. (Solanaceae) known as “henbane” in our country, is widely
distributed in Europe and Asia. In Turkey, henbane seeds have been used in folk
medicine to eradicate worms in the eyes. In the light of this information, antilusidal
activity of H. niger seeds, which had previously been proven to be effective against
different pathogens, was evaluated in this study.For this purpose, n-Hexane,
ethylacetate, methanol and alkaloid extracts were prepared from the seeds of the
plant and their larvicidal activities on Lucilia sericata larvae were evaluated. In the
present study,the development of the larvae was observed after application of H.
niger seeds extracts. EC50 and EC90 values of the alkaloid extract were calculated
and morphological abnormalities investigated. Alkaloid extract prepared from the
seeds of this plant displayed significant larvicidal activity and EC50 values were
found to be 8.04, 8.49, and 7.96g/mL against first, second, and third larval instar,
respectively. It was determined that malformations of larvae included damaged
larvae with small size, contraction and weak cuticle.Furthermore, high
performance liquid chromatography (HPLC) analysis was performed on alkaloid
extract of H. niger seeds and main components of the extract were determined. It
has been determined that the alkaloid extract mainly contain hyoscyamine and
scopolamine using HPLC. These results confirm the folkloric use of the plant and
suggest that the alkaloid content of the plant could be responsible for the larvicidal
activity.
Key Words : Black henbane, Hyoscyamus niger, Lucilia sericata,
Solanaceae, Myiasis