Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi Üniversitesi, Sağlık Bilimleri Enstitüsü, Turkey
Approval Date: 2014
Student: HAZAL DEMİR YARAR
Supervisor: SUNA ÖMEROĞLU
Abstract:In our study, pre-pubertal and pubertal period in rats with unilateral ligation of the ductus deferens later, testis and ductus deferens, structural and morphological changes occurring in the tissues was aimed to investigate.In the experiment, 6 male pre-pubertal and 6 male pubertal Wistar albino rats were used. And the subjects were divided into four groups. Subjects with unilateral ligation of the ductus deferens was performed. Untreated side were considered as control group. Following ligation in the second month, high doses were sacrificed under the anesthesia on expiry of the subjects were testis and ductus deferens tissues. Tissues were blocked passage of the histological tracking method. Section for hematoxylin-eosin and VEGF ımmunohistochemical staining was illustrated by applying paint. Testicular tissue and the seminiferous tubule diameter of seminiferous tubule epithelial thickness was analyzed statistically using Mann-Whitney U test with Bonferroni.In our study, unilateral ligation of the ductus deferens, both in pre-pubertal period and pubertal period, testis and ductus deferens tissues of rats lead to structural changes, morphological and statistically affect on spermatogenesis process has been, after ligation the seminiferous epithelium and the seminiferous tubule diameter were thickness , and ductus deferens epithelial structure and muscles in the structure of muscle fibers forming the base degenerative changes were observed.