Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi Üniversitesi, Fen Bilimleri Enstitüsü, Turkey
Approval Date: 2015
Student: LEYLA BİGEÇ
Supervisor: NESLİHAN GÜNDOĞAN BAKIRCI
Open Archive Collection: AVESIS Open Access Collection
Abstract:In this study, 100 fish samples (50 freshwater, 50 seawater) which are provided for consumption in Ankara, were used as material. Enterococcus species were isolated from fish samples and identified. Identified Enterococcus species were analysed for proteolytic activity at 4°C, 20°C and 37°C, lipolytic activity at 30°C, somevirulenceproperties as slime, biofilm,gelatinase, DNase,hemolysis andantibiotic resistance withKirby-Bauerdisk diffusion methodwere researched. The distribution rates among 150 Enterococcus isolated from fishes were %18,7Enterococcus spp., %16,7 E. casseliflavs, %14 E. flavescens., %13 E. gallinarum, %8,7 E. raffinosus, %8 E. solitarius, %7,3 E. pseudoavium, %4 E. avium, %4 E. faecalis %2 E. malodorats, %0,7 E. durans, %0,7 E. mundtii, %0,7 E. hirae, %0,7 E. dispar and %0.7 E. faecium.In our research, 102 (68%) slime (+), 19 (12,7%) gelatinase (+), 128 (85,3%) DNase (+) and 3 (2%) hemolyses (+) are in 150 Enterococcus isolated from the fish samples. By using microplate method all Enterococcus isolates after 24 hours of incubation on tryptone soy broth %18,7 strong biofilm, %52,7 medium positive, %24 weak positive and %1,3 negative biofilm formation have been identified. On the same isolates 2% glucose containing the TSB after 24 hours of incubation in %33,3 strong biofilm, %40 medium positive, %14,7 weak positive and %9,3 negative biofilm formation have been identified. The distribution of antibiotic resistances of Enterococcus isolates (freshwater fish samples) was (32.9 %)erythromycin, (35.6%) ciprofloxacin, (83.6%) ampicillin, (54.8%) tetracycline, (56.2%) vankomycin, (24.7%) nitrofurantoin, (93.2%)novobiocin, (19.2%) gentamicin, (24.6%) teicoplanin, (28.8%) chloramphenicol. The distribution of antibiotic resistances of Enterococcus isolates (seawater fish samples) was (64.9%)erythromycin, (49.3%) ciprofloxacin, (92.2%) ampicillin, (68.8%) tetracycline, (44.1%) vankomycin, (35.1%) nitrofurantoin, (44.1%)novobiocin, (20.8%) gentamicin, (3.9%) teicoplanin, (31.2%) chloramphenicol.From 150 Enterococcus isolates, 24 of the isolates (16%) strong positive, 73 of the isolates (48,7%) medium positive, 26 of the isolates (17,3%) weak positive have been identified proteolytic activity at 4°C. 31 of the isolates (20.7 %) strong positive,13 of isolates (8.7%) medium positive, 4 of the isolates (2.7%) weak positive have been identified proteolytic activity at 20°C. 42 of the isolates (28%) strong positive, 42 of isolates (28%) medium positive, 38 of the isolates (25.3%) weak positive have been identified proteolytic activity at 37°C.None of Enterococcus isolate were shown 30 °C lipolytic enzyme activity.