Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi Üniversitesi, Fen Bilimleri Enstitüsü, Turkey
Approval Date: 2009
Student: SEVCAN MAMUR
Supervisor: DENİZ YÜZBAŞIOĞLU
Abstract:In this study, the in vitro genotoxic effects of sodium sorbate and potassium sorbate which are frequently used in foods as antimicrobials, have been determined in human peripheral blood lymphocytes by using chromosome aberrations (CAs), sister chromatid exchanges (SCEs), micronuclei (MN) and comet assay. 100, 200, 400 and 800 μg/ml concentrations of sodium sorbate and 125, 250, 500 and 1000 μg/ml concentrations of potassium sorbate were used. Both food additives used in this study sifnificantly increased the frequency of chromosome aberrations and sister chromatid exchanges compared with the negative control. Micronuclei frequency statistically increased in 400 and 800 μg/ml concentration of sodium sorbate. Hovewer potassium sorbate did not effect the micronuclei frequency in human lymphocytes culture. 200 μg/ml concentration of sodium sorbate and 1000 μg/ml concentration of potassium sorbate significantly decreased the mitotic index compared with the negative control. Replication and nuclear division indices were not effected by the sodium sorbate and potassium sorbate. According to the comet assay results, primary DNA damage significantly increased by all treatments of both additives. The comet tail intensity and comet tail length (expect 400 μg/ml for tail lenght for sodium sorbate) significantly increased compared with the control in all treatments. The results of experiments showed that the additives used in this study have clastogenic and mutagenic and also DNA damaging effects in cultured and isolated human lymphocytes.