Research Information System
Thesis Type: Doctorate
Institution Of The Thesis: Gazi University, Sağlık Bilimleri Enstitüsü, Farmakognozi Anabilim Dalı, Turkey
Approval Date: 2019
Thesis Language: Turkish
Student: MERT İLHAN
Supervisor: Esra Akkol
Abstract:
There are numerous plants which are used for the treatment of gynecological disorders in
ethnobotanical studies conducted in Turkey. The present study aimed to investigate the effect
of Anthemis austriaca Jacq., Cichorium intybus L., Melilotus officinalis (L.) Pall., Mentha
longifolia (L.) Huds. and Urtica dioica L. on endometriosis and polycystic ovary syndrome
(PCOS). For this purpose, n-hexane, ethyl acetate (EtOAc) and methanol (MeOH) extracts
were prepared from the flowers of A. austriaca and the aerial parts of C. intybus, M.
officinalis, M. longifolia and U. dioica, successively. The effects of the prepared extracts
were evaluated by surgically-induced endometriosis and letrozole-induced PCOS rat
models. Since the extracts prepared from A. austriaca showed the highest activities in
endometriosis and PCOS rat models, it was decided to conduct bioactivity guided
fractionation technique on that plant. The MeOH extract, which had the highest activity in
endometriosis rat model, was applied to the vacuum liquid chromatography using RP-18 for
the fractionation. The obtained fractions were combined according to their thin layer
chromatography profiles in order to gain 4 main fractions [Fr. (1-6); Fr. (7-13); Fr. (14-20);
Fr. (21-29)]. When the activities of these fractions were evaluated in endometriosis rat
model, Frs. (1-6) and (14-20) were found to have statistically significant activity. Further, 4-
(β-D-glucopyranosyloxy)-6-methyl-2H-pyran-2-one was isolated from Fr. (1-6), quercetin,
apigenin-7-O-(3''-acetyl)-β-D-glucopyranoside, apigenin-7-O-(6''-acetyl)-β-Dglucopyranoside,
apigenin-7-O-β-D-glucopyranoside and quercetin-7-O-β-Dglucopyranoside
were isolated from Fr. (14-20) which had the highest activity among the
fractions. The n-hexane extract of A. austriaca was found to have the highest activity in
letrozole-induced PCOS rat model. Further phytochemical studies on the n-hexane extract,
β-amyrin palmitate, taraxasterol acetate and taraxasterol, which are triterpene derivatives,
were isolated from n-hexane extract of A. austriaca. Consequently, the efficacy of A.
austriaca against endometriosis could be due to its flavonoids, whereas its effect against
PCOS could be due to its triterpene derivatives.
Key Words : P h a rmacognosy, Ethnopharmacology, Asteraceae, Anthemis austriaca,
Endometriosis, PCOS, Flavonoid, Triterpene