Research Information System
Thesis Type: Expertise In Medicine
Institution Of The Thesis: Gazi Üniversitesi, Tıp Fakültesi, Turkey
Approval Date: 2018
Thesis Language: Turkish
Student: ZEYNEP KOÇ
Supervisor: IŞIL FİDAN
Abstract:Determination of IL-28B polymorphism and relationship between viral load in hepatitis B and C infections in Turkish Population Hepatitis B virus (HBV) and hepatitis C virus (HCV) infections are causing to health problems that can lead to important clinical presentations with causing acute or chronic inflammation of the liver. In HBV and HCV infected patients, it is important to determine the factors that lead to chronicity in HBV and HCV infection so that we can take measures to prevent chronic infection. Recently, the host factors single nucleotide polymorphisms (SNP) in cytokine genes that affect the clinical course of the infections become important. In this study, we aimed to investigate the frequency of Interleukin-28B (IL-28B) rs12979860, rs8099917 and rs12980275 SNP in HBV and HCV infection, to compare different clinical presentations with control to analyze haplotypes, to detect IL-28B plasma levels and relationship between the SNPs genotypes and IL-28B plasma levels among Turkish patients. IL-28B SNPs were investigated by Real Time PCR. Plasma levels of IL-28B were measured by ELISA. In the study, the rs12979860CT genotype and T allele and the rs12980275AG genotype and A allele were significantly lower in patients with chronic HBV infected patients when compared with controls. The rs12979860CC and rs12980275AA genotype were significantly higher in chronic HBV infected patients when compared with controls. However, the TG genotype and G allele 168 frequencies of the rs8099917 in patients with HCV infection and rs8099917TT genotype and T allele in controls were significantly higher. According to haplotype analysis, the TGG haplotype was found significantly higher in patients with HCV infection compared with controls. The plasma IL-28B levels were higher in controls compared to patients with HBV or HCV infection. In the HBV infected patients with rs12980275AG genotype detected plasma IL-28B levels significantly higher compared to other genotypes. In conclusion, we believe that IL-28B SNPs could be used as biomarkers for predicting clinical outcomes and be useful to take precautions to prevent progression of hepatitis infection and to improve new gene therapies with further investigations.