Research Information System
Thesis Type: Doctorate
Institution Of The Thesis: Gazi Üniversitesi, Sağlık Bilimleri Enstitüsü, Turkey
Approval Date: 2015
Student: SÜHAN GÜRBÜZ
Supervisor: ALTAN DOĞAN
Abstract:A novel prototype membrane has been designed and fabricated with a graded structure (polycaprolactone- PCL, collagen- COL, nanohydroxyapatite- nHA) by combining recently emerging periodontal tissue engineering field with guided tissue regeneration principles. The purpose of thesis study is to research effect of the triphasic membranes, which are planned to serve hierarchically on MC3T3-E1 cells, to evaluate nHA for bone regeneration and to release kinetics of the membrane loaded with rhBMP-7. SEM, EDX, FTIR-ATR and release studies of the membranes which defines membranes physical properties have been performed. As a result of these studies, it has been determined nHA and collagen joined the membrane successfully, the core layer had a suitable size for osteoblasts (337 nm). It was also discovered that 42% of rhBMP-7 was released during the first 4 hours and the remainder was released during the next three days. In SEM images, cells adhere to even at same places penetrate into the inner layer of the membrane and ECM formed. It was observed through MTT analysis that vitality of the cells within the membranes as well as greatest rate of proliferation occured in presence of rhBMP-7. Osteoblastic differentiation markers have been determined through ALP analysis and osteogenic gene results through real time RT-qPCR. ALP activity COL-I, Runx2, OCN, OPN gene levels have been found significantly higher in BMP-7 group. In conclusion, the data we obtained in three different prototype membranes, the membrane laden with rhBMP-7 and containing PCL, nHA, COL can be an effective membrane in periodontal tissue regeneration.