Research Information System
Thesis Type: Doctorate
Institution Of The Thesis: Gazi University, Sağlık Bilimleri Fakültesi, HİSTOLOJİ VE EMBRİYOLOJİ ANABİLİM DALI, Turkey
Approval Date: 2021
Thesis Language: Turkish
Student: İsmail TÜRKOĞLU
Supervisor: Suna Ömeroğlu
Abstract:
Ovarian hyperstimulation syndrome (OHSS) is a serious iatrogenic complication that can
occur during assisted reproductive techniques. There is no specific treatment for OHSS.
More research is needed to elucidate the pathophysiology behind the condition and to
find a permanent treatment for it. In this study, it was aimed to investigate the effects of
quercetin on ovarian morphology and related VEGF/VEGFR-2 expression and IL-6 level rat
model of OHSS. 28 immature female Wistar Albino rats, 21-22 days old, were randomly
divided into 4 groups (Control, KOH, OHSS and OHSS+Quercetin), 7 in each group.
Hematoxylin-Eosin, Periodic acid Schift staining for histo-morphological examination of
ovarian tissue and immunohistochemical staining for evaluation of VEGFA and VEGFR-2
expression, ELISA analyzes to determine the concentration of IL-6, VEGFA in the
peritoneal fluid and E2 in the serum were performed. Body weight, ovarian weight and
diameters, vascular endothelial growth factor (VEGF) and IL-6 levels of peritoneal fluid,
serum E2 levels, and ovarian expression of VEGFA and VEGFR-2 were compared between
the groups. Ovarian weights and diameters, peritoneal fluid levels of VEGFA and IL-6 and
ovarian VEGFA, VEGFR-2 expression were found to be significantly lower in quercetintreated
rats than in the untreated OHSS group. In addition, when the structures in the
ovary were evaluated, it was found that quercetin administration significantly decreased
the number of corpus luteum and cystic structures compared to the group with OHSS,
and increased the number of atretic follicles. It was determined that quercetin has effects
that reduce OHSS findings. It has been demonstrated that the use of quercetin can be
effective both in the prevention and control of OHSS
Key Words : OHSS, ovary, quercetin, vegf, vegfr-2, IL-6