Research Information System
Thesis Type: Doctorate
Institution Of The Thesis: Gazi University, Turkey
Approval Date: 2016
Thesis Language: Turkish
Student: Hilal Torul
Supervisor: UĞUR TAMER
Open Archive Collection: AVESIS Open Access Collection
Abstract:In this thesis, determination of glucose and cancer biomarker was carried out by using microfluidic chips in blood samples. For this purpose, three different types of chip were prepared and they were used to detect two different target molecules in blood sample. Moreover, in the scope of carried out thesis, a smartphone based fluorescent microscope was created to relate hand-held systems. The determination of glucose molecules was achieved by using self-assembled monolayers (SAMs) and paper based micro fluidic assays in blood sample and also the detection of carcinoembryonic antigen (CEA) was achieved using lateral flow test strips. As the first study of this thesis, a new detection method for blood glucose was developed using a Surface Enhanced Raman Scattering (SERS) probe (4- mercaptophenylboronic acid (4-MBA)/1-decanethiol (1- DT)) embedded in selfassembled monolayers (SAMs). The analytical performance was evaluated and linear calibration graphs were obtained in the glucose concentration range of 2 16 mM, which is also in the range of the blood glucose levels, and the detection limit was found to be 0,5 mM in reference blood sample. The glucose concentration was found to be 5,61 ± 0,29 mM in the reference blood sample and the certified value for glucose was 6,17 ± 0,11 mM. The recovery of the glucose in the reference blood sample was found as 90,9 %. As the second study of this thesis, a paper membrane-based SERS platform for the determination of blood glucose level was developed using a nitrocellulose membrane as substrate paper, and the microfluidic channel was simply constructed by wax-printing method. The rod shaped gold nanoparticles were modified with 4-MBA and 1-DT molecules and used as embedded SERS probe for paper based microfluidics. Total analysis was completed in 5 min. In SERS measurements, the intensity of the band at 1070 cm−1 which is attributed to B OH vibration decreased depending on the rise in glucose concentration in the blood sample. The glucose concentration in the reference blood sample was found to be 5,43 ± 0,51 mM and the recovery of the glucose was found as 88 %. Ultimately, in the scope of carried out thesis, the detection of CEA was achieved from whole blood using smartphone based fluorescence microscope with quantum dot-based lateral flow immunoassay test strip. To design smartphone based fluorescence microscope, commercial Nokia Lumia 1020 was used as a cell phone. The lateral flow immunoassay which is on the basis of a sandwich immunoreaction was constructed by creating test and control lines on nitro cellulose membrane. Under optimal conditions, the LOD of CEA protein was found to be 1,0 ng/ml by using developed lateral flow assay with only 80 μL sample volume.