Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi University, Sağlık Bilimleri Enstitüsü, Turkey
Approval Date: 2009
Thesis Language: Turkish
Student: Seda Usta
Supervisor: IŞIL FİDAN
Abstract:More widespread use of broad-spactrum antibiotics, organ transplants are much more frequent causes, such as supressed immune systems of patients and such patients increases everyday to show the presence Candidiasis. In which immunity aganist Candida infections are many factors play a role. Cytokines are one of them. In our study, immunological factors that occur during Candidiasis the determination of different cytokine profiles which is intended. IL-17 cytokine expression in kidney tissues of infected rats to Real-Time PCR assay was determined. Infection model with was carried out 10231 ATCC reference strains of C.albicans given to rats. In addition, a control group was formed in order to compare with infected group. Culture that was made from kidney tissue taken from animals was found positive. Candidiasis formation on kidney tissues is shown by working with Real-Time PCR assay. The presence of fungus infection was confirmed by using specific primers. As a result of PCR studies Candidiasis group IL-17, ten out of twelve examples (83.3%) were found positive. None of samples in the control group was found positive. Accordingly, during Candidiasis immune cells play an important role in the relationship of cytokines. Fully determination of effective cytokine profile is intended to allow new development means of protection and treatment of Candida infections.