Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi Üniversitesi, Fen Bilimleri Enstitüsü, Turkey
Approval Date: 2014
Student: ESRA ERİKEL
Supervisor: DENİZ YÜZBAŞIOĞLU
Abstract:Cynarin and luteolin are plant phytochemicals that possess a variety of chemical and biological activities, including free-radical scavenging and antioxidant activity. This study was performed to determine in vitro genotoxic and antigenotoxic effects of cynarin and luteolin against MMC (Mitomycin C, an antineoplastic agent) and H2O2 (Hydrogen peroxide) induced genomic instability in human peripheral blood lymphocytes. Four different genotoxicity assay was carried out, including chromosomal aberration (CA), sister chromatid exchange (SCE), micronucleus (MN) and comet assay. The lymphocytes were incubated with different concentrations of cynarin (6,25; 12,50; 25,00; 50,00 and 100,00 µg/mL) and luteolin (0,39; 0,78; 1,56; 3,12 and 6,25 µg/mL) alone and simultaneously with 0,20 µg/mL MMC. Cynarin and luteolin did not significantly showed genotoxic effects in the CA, MN and comet assays. Moreover, SCE frequency was not significantly enhanced by cynarin (except 100 µg/mL) and luteolin. On the other hand, both phytochemical reduced CA, SCE and MN frequency induced by MMC and also significantly decreased primary DNA damage at all concentrations induced by H2O2. The highest concentrations of both phytochemicals alone significantly decreased mitotic index however all treatments increased the reduction of MI induced by MMC. The results demonstrated that cynarin and luteolin exhibited antigenotoxic effects rather than inducing genotoxic effects. It is believed that both phytochemicals can act as potential chemopreventive compounds against genotoxic agents.