Evaluatıon Of Epıthelıal Mesenchymal Transıtıon Markers In Ameloblastoma And Odontogenıc Keratocysts


Thesis Type: Doctorate

Institution Of The Thesis: Gazi Üniversitesi, Sağlık Bilimleri Enstitüsü, Turkey

Approval Date: 2016

Student: FARID MUSEYIBOV

Supervisor: BENAY YILDIRIM

Abstract:

Epithelial mesenchymal transition (EMT) is a multiple-stage morphogenetic phenomenon where epithelial cells lose their epithelial characteristics and gain mesenchymal characteristics. Recently conducted studies show that EMT plays an important role not only in tumor invasion, but also in tumor recurrence and biology. The potential role of EMG molecules in the tumorigenesis of odontogenic neoplasms has started to be studied recently. In this study, our objective is to assess the expression of Snail; Integrin-β-1; Twist and E-cadherin in ameloblastomas and keratocystic odontogenic tumors and ortho-keratotic type odontogenic keratocysts. Twentyone cases of ameloblastoma (ABL), 31 cases of keratocystic odontogenic tumor (KOT); eight cases of odontogenic keratocysts showing ortho-keratinisation (OKC) and as a control group 10 dental follicle tissues (DF) were included in this study from the archives of Gazi University Faculty of Dentistry, Department of Oral Pathology between the years 2007-2014. All the cases have been assessed after being stained with twist, snail, e-kaderin and integrin β-1 antibodies by using the immunohistochemistry method. The snail positivity observed in ABL was statistically significantly higher compared to KOT and OKC (p≤0,05). The highest twist expression has been observed in ameloblastomes in 61% of cases. However, a statistically significant difference could not be determined between ABL, KOT, OKC and DF groups in what regards twist positiveness. No statistically meaningful difference has been found between the twist staining scores of KOT and OKC cases. The loweste-cadherin expression has been determined in ABL cases as 33%. Similarly, the lowest staining score average has been found in ABL cases (0,71). Both results are statistically lower compared with the DF group (p≤0,05). The e- cadherinstaining score observed in the KOT group is statistically significantly lower than the DF group (p≤0.05). The integrin β-1 pozitiveness ratio of the ABL group has been observed as significantly lower than the KOT, OKC and DF groups (p≤0,5). The integrin β-1 staining scores in ABL and KOT groups are significantly lower than those in the DF group (p≤0,5). There was not a statistical significant difference in solid and unicystic subtypes of ameloblastoma in terms of EMT protein expressions. Our results, although, suggested a role of EMT in pathogenesis and biology of odontogenic tumours.