Virulence factors and antibiotic resistance of Enterococcus isolated from clinical and food samples


Thesis Type: Postgraduate

Institution Of The Thesis: Gazi University, Fen Bilimleri Enstitüsü, Turkey

Approval Date: 2013

Thesis Language: Turkish

Student: Fatma Öztürk Torlak

Supervisor: NESLİHAN GÜNDOĞAN

Open Archive Collection: AVESIS Open Access Collection

Abstract:

In this study, 125 food samples collected in a variety of Ankara?s markets (25 hind-limb, 25 mince, 25 milk, 25 ice cream and 25 cheese) and 125 clinical samples (blood and urine) collected from several hospitals were used as material. In studied 125 food samples Enterococcus isolation of 100, and from 125 clinical samples 101 Enterococcus were isolatation is done. Food samples and a variety of Enterococcus species isolated from clinical specimens were identified by biochemical tests. in the defined ısolates some virulence properties as slime, gelatinase, DNase, hemolysis, and antibiotic resistance with Kirby-Bauer disk diffusion method were researched. 55 (55%) E. faecalis, 30 (30%) E. faecium, 1 (1%) E.gallinarum, 6 (6%) E. mundtii, 2 (2%) E. durans, 1 (1%) E. raffinosus and 5 (5%) Enterococcus spp. are defined like this in 100 Enterococcus isolates ısolated from the food samples 50 (49,5%) E. faecalis, 45 (44,5%) E. faecium, 2 (2%) E. gallinarum, 1 (1%) E. durans, 1 (1%) E. casseliflavus, 1 (1%) E. avium ve 1 (1%) E. hirae are defined as above in 101 Enterococcus isolates isolated from the clinical samples. In our research, 35 (35%) slime (+), 10 (10%) gelatinase (+), 6 (6%) DNaz (+) and 8 (8%) hemolyses (+) are in 100 Enterococcus isolates isolated from the food samples. 40 (39,6%) slime (+), 15 (14,9%) gelatinase (+), 16(15,8%) DNaz (+) and 12(11,9%) hemolyses (+) are in 101 Enterococcus isolates ısolated from clinical samples 46 (46%) of 100 Enterococcus isolates isolated from the food samples are found resistant to erythromycin, 12 (12%) of them are resistant to gentamicin, 6 (6%) of them are resistant to penicillin, 5 (5%) are resistant to teicoplanin, 3 (3%) are resistant to ampiciline, 2 (2%) are resistant to chloramphenicol and 1 (1%) are resistant to levofloxacin. 55 (54,5%) of 101 Enterococcus isolates isolated from clinical samples are found resistant to erythromycin, 15 (%14,9) are resistant to gentamicin, 12 (10.9%) are resistant to penicillin, 12(%11.9) are resistant to chloramphenicol, 11 (10.9%) to levofloxacin, 11 (10.9%) to ampiciline and 7 (6.9%) to teicoplanin. In Enterococcus varieties ısolated from food samples and clinical samples the ısolate which is resistant to vancomycin are not determined.