Analysıs Systems For Total Proteın Assays Based On Nanopartıcles

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Thesis Type: Postgraduate

Institution Of The Thesis: Gazi University, Turkey

Approval Date: 2014

Thesis Language: Turkish

Student: Merve Eryılmaz

Supervisor: UĞUR TAMER

Open Archive Collection: AVESIS Open Access Collection

Abstract:

The quantitative determination of total protein is an important analytical task for the life sciences. Especially, accurate quantification of total proteins of milk has importance in many applications, ranging from food quality to diagnosis of diseases. In this scope, the determination of total protein using Surface enhanced Raman Spectrocopy (SERS) is proposed. For this purpose, two different methods were developed. Firstly, we prepared azide modified silica surfaces/particles and 5,5'dithiobis-2-nitrobenzoic acid (DTNB)-alkyne modified gold nanorod particles to perform both a heterogen assay and a homogen assay. In these assays, biuret reagent added to the medium to occur the click reaction between the particles in the presence of protein. The protein concentration was monitored in a paralel signal ratio of DTNB tag. In the second method, o-phthalaldehyde (OPA) used to quantify total protein using nanoparticles. Completed OPA reagent binds specifically only to amine groups of proteins. Proportional to the increasing amount of protein concentration, OPA molecules spectral band intensity were monitored and linear calibration graph was obtained. In developed methods, bovine serum albumine (BSA) was used as protein standard and as a real sample, milk was tested only by dilution