Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi University, Turkey
Approval Date: 2015
Thesis Language: Turkish
Student: Ethem Serhat YAVAŞ
Supervisor: NESLİHAN GÜNDOĞAN
Open Archive Collection: AVESIS Open Access Collection
Abstract:In this study 206 Enterobacteriaceae isolates, from 112 food samples sold in several markets in Ankara were used as materials. Amino acid decarboxylase activitiy, biogenic amine production capabilities, biofilm measurement by using microplate method and the extended spectrum beta- lactamase activities and the antibiotic resistances of the isolates, conducted by using double disk synergy and Kirby-Bauer disk diffusion method of these isolates have been investigated respectively. The distribution rates among 206 Enterobacteriaceae strains isolated from foods were 26,2% Escherichia spp. , 18,9% Klebsiella spp. , 22,8% Enterobacter spp. , 9,2% Hafnia spp. , 8,8% Citrobacter spp. , 7,3% Serratia spp. , 2,9% Proteus spp. , 1,5% Pantoea spp. , 1,5% Providencia spp. , 0,9% Morganella spp. . 27 (13.1%) histidine, 149 (72.3%) lysine, 145 (70.3%), ornithine and 109 (52.9%) arginine positive amino acid decarboxylase activity, 126's (61.1%) of histidine, 200 (97%) lysine, 203 (98.5%) ornithine, 205 (99.5%) arginine biogenic amine productions of these 206 isolates of Enterobacteriaceae gave positive results. By using microplate method, 6,4% of Enterobacteriaceae isolates showed strong biofilm formation, after 24 hours of incubation in Tryptic Soy Broth (TSB) and 4% of Enterobacteriaceae isolates showed strong biofilm formation after 24 hours in TSB+1% Glucose. 112 (54.3%) of Enterobacteriaceae isolates gave positive results in the slime. In Enterobacteriaceae strains the highest antibiotic resistance were seen against amoxicillin-clavulanic acid (64,5%), ampicillin-sulbactam (49,5%), amikacin (22,8%), chloramphenicol (20,3%). 65 (31,5%) of 206 Enterobacteriaceae isolates showed positive results as extended spectrum beta lactamase (ESBL). The rates of 65 ESBL (+) Enterobacteriaceae strains were 40% E. coli, 16,9% K. oxytoca, 4,6% K. pneumoniae, 4,6% E. cloacae, 4,6% H. alvei, 9,3% C. freundii, 4,6% S. marcescens, 1,5% S. fonticola, 3,2% P. agglomerans, 4,6% P. vulgaris, 1,5% E. sakazakii, 4,6% C. amalonaticus. The highest resistance rates of ESBL (+) Enterobacteriaceae strains were against amoxicillin-clavulanic acid (58,5%), ampicillin-sulbactam (44,7%), amikacin (30,8%), tetracycline (24,7%) and piperacillin-tazobactam (23,1%).