Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Gazi University, Fen Fakültesi, Biyoloji, Turkey
Approval Date: 2021
Thesis Language: Turkish
Student: Busenaz Tuncay
Co-Supervisor: Çiğdem Alev Özel
Supervisor: Deniz Yüzbaşıoğlu
Abstract:Vermicompost is obtained as a result of vermiculture studies carried out to turn various organic wastes into nutritious "organic fertilizers" by worms. Tissue culture performed with the nutritious culture medium and controlled aseptic conditions for the growth of plant cells, tissues, and organs. This study aimed to investigate the genotoxic and antigenotoxic effects of vermicompost during 24 and 48 hours on Allium test in tissue culture. Three different protocols were applied to the root tips; 1) A. cepa root tips were treated with 0, 5000, 10000, 20000, 40000 and 80000 μg/mL of vermicompost for 24 and 48 hours to determine its genotoxic potential. 2) A.cepa root tips were treated with the same concentrations of vermicompost for 23 and 47 hours and H2O2 for the last 1 hour to determine their antigenotoxic potential (pretreatment). 3) A.cepa root tips were also treated with the H2O2 for the first 1 h and then same concentrations of vermicompost for 23 and 47 hours in the presence (post-treatment). As a result almosts all concentrations of vermicompost were significantly increased the mitotic index (MI) at 24 h (except 5000 and 20000 μg / mL) and 48 h treatments compared with the negative control. In addition, vermicompost treatments did not significantly increase the mitotic abnormalities. In antigenotoxicity test protokol, all the concentrations of pre- and post-treatment of vermicompost increased the frequency of MI in root tip cells at both treatment periods. Furthermore all the concentrations of pre- and post-treatment of vermicompost decreased the frequency of mitotic abnormalities induced by H2O2. These results show that while vermicompost treatment alone does not induce genotoxic effects, and vermicompost revealed protective effect agaist H2O2. This study indicated antigenotoxic potential of vermicompost, which has been extensively used in traditional agriculture, against genotoxic chemicals. This is the first research indicating that vermicompost can be used in tissue culture conditions