Akademik Veri Yönetim Sistemi
Yükseköğretim Kurumları Destekli Proje, 2015 - 2017
Some single nucleotide polymorphisms
(SNPs) of the gene encoding interleukin 28B (IL28B) may increase the human susceptibility to getting infected
with microorganisms. In our study, we aimed to investigate the prevalence of
rs12979860, rs8099917 and rs12980275 in the IL28B
in patients with Hepatitis B Virus (HBV) or Hepatitis C Virus (HCV) infection
and to determine the relationship of these polymorphisms with plasma IL28B
levels. For this purpose, HBV-infected 64, HCV-infected 66 patients and 70
healthy individuals were included in the study. The SNPs were investigated by
real time PCR (Polimerase Chain Reaction, RT-PCR) using TaqMan SNP Genotyping Assay.
The plasma levels of IL28B were measured by ELISA.
The frequencies of the rs12980275AG genotype and G
allele (p=0.003 and p=0.04, respectively), and the rs12979860CT genotype and T
allele (p=0.01 and p=0.04, respectively) were lower in HBV-infected patients. In
HCV-infected patients, the rs8099917TG genotype and G allele frequencies (p=0.04)
were higher and the TGG haplotype showed a statistically significant difference
(p=0.04). The mean IL28B plasma levels were higher in the control group than in
the HBV or HCV-infected patient groups (p=0.001 and p=0.01, respectively). However,
HBV-infected patients with the rs12980275AG genotype showed a significant
difference in plasma IL28B levels compared to other genotypes (p=0.0001) and
these patients had lower viral loads (<105 IU/ml).
According to all these results, the rs12979860CT and
rs12980275AG genotypes may play a role in preventing the chronicity of HBV
infection, and the rs8099917TG genotype may contribute the conversion to
chronic HCV infection. The presence of G allele in rs8099917 polymorphism can
be considered as a risk allele for chronic HCV infection, and TGG haplotype may
have a strong predictive effect in increasing susceptibility to chronic HCV
infection. It is recommended
to evaluate the genotypic distribution of IL28B before treatment because of its
prognostic significance in HBV or HCV infected patients. In HBV infection, the
rs12980275AG genotype which is thought to have a protective effect by limiting viral
replication with increased plasma IL28B levels, can be used as a good
prognostic factor. These polymorphisms could be used as biomarkers to predict
the clinical consequences of HBV or HCV infected patients, and to take
precautions to prevent the infection from becoming chronic and its
complications, and to develop new molecular targeted therapies with further
research.