Türk toplumunda Hepatit B ve Hepatit C enfeksiyonunda IL-28B polimorfizminin ve viral yük ile ilişkisinin belirlenmesi


Tuğ E., Fidan I. (Yürütücü)

Yükseköğretim Kurumları Destekli Proje, 2015 - 2017

  • Proje Türü: Yükseköğretim Kurumları Destekli Proje
  • Başlama Tarihi: Ocak 2015
  • Bitiş Tarihi: Şubat 2017

Proje Özeti

Some single nucleotide polymorphisms (SNPs) of the gene encoding interleukin 28B (IL28B) may increase the human susceptibility to getting infected with microorganisms. In our study, we aimed to investigate the prevalence of rs12979860, rs8099917 and rs12980275 in the IL28B in patients with Hepatitis B Virus (HBV) or Hepatitis C Virus (HCV) infection and to determine the relationship of these polymorphisms with plasma IL28B levels. For this purpose, HBV-infected 64, HCV-infected 66 patients and 70 healthy individuals were included in the study. The SNPs were investigated by real time PCR (Polimerase Chain Reaction, RT-PCR) using TaqMan SNP Genotyping Assay. The plasma levels of IL28B were measured by ELISA.

The frequencies of the rs12980275AG genotype and G allele (p=0.003 and p=0.04, respectively), and the rs12979860CT genotype and T allele (p=0.01 and p=0.04, respectively) were lower in HBV-infected patients. In HCV-infected patients, the rs8099917TG genotype and G allele frequencies (p=0.04) were higher and the TGG haplotype showed a statistically significant difference (p=0.04). The mean IL28B plasma levels were higher in the control group than in the HBV or HCV-infected patient groups (p=0.001 and p=0.01, respectively). However, HBV-infected patients with the rs12980275AG genotype showed a significant difference in plasma IL28B levels compared to other genotypes (p=0.0001) and these patients had lower viral loads (<105 IU/ml).

According to all these results, the rs12979860CT and rs12980275AG genotypes may play a role in preventing the chronicity of HBV infection, and the rs8099917TG genotype may contribute the conversion to chronic HCV infection. The presence of G allele in rs8099917 polymorphism can be considered as a risk allele for chronic HCV infection, and TGG haplotype may have a strong predictive effect in increasing susceptibility to chronic HCV infection. It is recommended to evaluate the genotypic distribution of IL28B before treatment because of its prognostic significance in HBV or HCV infected patients. In HBV infection, the rs12980275AG genotype which is thought to have a protective effect by limiting viral replication with increased plasma IL28B levels, can be used as a good prognostic factor. These polymorphisms could be used as biomarkers to predict the clinical consequences of HBV or HCV infected patients, and to take precautions to prevent the infection from becoming chronic and its complications, and to develop new molecular targeted therapies with further research.