Clustering of nosocomial Candida kefyr infections among hematological patients in a university hospital: Molecular typing of the strains by PFGE and RAPD

Kalkanci A., Saracli M. A., Guzel Ö., Yildiran S. T., Senol E., Kustimur S.

JOURNAL DE MYCOLOGIE MEDICALE, cilt.17, sa.4, ss.250-255, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 4
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.mycmed.2007.09.003
  • Dergi Adı: JOURNAL DE MYCOLOGIE MEDICALE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.250-255
  • Anahtar Kelimeler: Candida kefyr, hematology, nososcomial, infection, AP-PCR, PFGE, RAPD, INVASIVE FUNGAL-INFECTIONS, IN-VITRO ACTIVITY, AMPHOTERICIN-B, ALBICANS, IDENTIFICATION, CANCER, EPIDEMIOLOGY, CASPOFUNGIN, FLUCONAZOLE, PATHOGEN
  • Gazi Üniversitesi Adresli: Evet

Özet

This study spreading over a span of eight months described Candida kefyr infections in hematolology patients from a Turkish University hospital. A total of 18 strains were isolated from various body locations of 18 patients. DNA fingerprinting of the isolates was performed by using randomly amplified polymorphic DNA (RAPD) method and karyotyping by pulsed-field get electrophoresis (PFGE). RAPD analysis of all the C. kefyr strains revealed that strains collected from 18 different patients exhibited seven different genotypes, while those were six in PFGE. Taken together, this is probably the first molecular survey of C. kefyr infections in the hematology unit documenting nosocomial transmission of C. kefyr isolates. (C) 2007 Elsevier Masson SAS. All rights reserved.