Preparation of carbon paste electrode containing polyaniline-activated carbon composite for amperometric detection of phenol

Arslan H., Senarslan D., Cevrimli B. S., Zengin H., Uzun D., Arslan F.

Bulgarian Chemical Communications, vol.50, no.1, pp.16-20, 2018 (Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 50 Issue: 1
  • Publication Date: 2018
  • Journal Name: Bulgarian Chemical Communications
  • Journal Indexes: Scopus
  • Page Numbers: pp.16-20
  • Keywords: Phenol, polyphenol oxidase, biosensor, polyaniline (pani), polyaniline activated carbon composite, carbon paste, POLYPHENOL OXIDASE, TYROSINASE BIOSENSOR, ENZYME ELECTRODE, WINES
  • Gazi University Affiliated: Yes

Abstract

© 2018 Bulgarian Academy of Sciences, Union of Chemists in Bulgaria.In this study, a novel carbon paste electrode was prepared using the salt form of polyaniline (pani)-activated carbon composite sensitive to phenol. Polyphenol oxidase enzyme was immobilized to the modified carbon paste electrode by cross-linking with glutaraldehyde. The amperometric determination is based on the electrochemical reduction of o-quinone generated in the enzymatic reaction of phenol at -0.15 V vs. Ag/AgCl. The effects of pH and temperature were investigated and optimum values were found to be 8.0 and 45 °C, respectively. The linear working range of the electrode was 1.0×10-6 - 5.0×10-5 M, R2 =0.9819. The storage stability and operation stability of the enzyme electrode were also studied.