Akademik Veri Yönetim Sistemi
JOURNAL OF SOLID STATE ELECTROCHEMISTRY, cilt.26, sa.2, ss.549-557, 2022 (SCI-Expanded)
In this study, a new amperometric biosensor for creatinine determination was developed. For this purpose, a polypyrrolepolyvinylsulfonate film was prepared by electropolymerization of pyrrole in a polyvinylsulfonate medium on a platinum plate. Creatinase and sarcosine oxidase enzymes were immobilized on polypyrrole-polyvinylsulfonate film by cross-linking with glutaraldehyde. The determination of creatinine was made based on the oxidation of hydrogen peroxide at 0.4 V formed as a result of the enzymatic reaction on the surface of the prepared biosensor. The linear working range of the biosensor obtained was found between 5.0 x 10(-6) and 1.0 x 10(-4) M. Using this linear graph, the Km (observed) and Imax (observed) values for the double enzyme electrode system were calculated as 5.0 x 10(-3) mM and 0.34 mu A/min, respectively. Optimum working pH and temperature were determined as 8.0 and 60 degrees C, respectively. The reusability and shelf life of the biosensor were determined. The effects of interferences in biological environments on biosensor response were investigated. For this purpose, uric acid, ascorbic acid, paracetamol, glycine, urea, and formaldehyde were used. The results have shown that the prepared biosensor has the potential to be used for creatinine determination in biological fluids.