Akademik Veri Yönetim Sistemi
The International Alumınıum-Themed Engineering and Natural Sciences Conference in Seydişehir/TURKEY (IATENS’19), October 4-6 2019, Konya, Türkiye, 4 - 06 Nisan 2019, ss.202-206
Juniperus L. (Cupressaceae) has been used since the ancient time for medical purposes such as cold
cough and wound healing treatments. It has almost 70 species around the world and Turkey has 7
species (J. drupacea Lab., J. communis L., J. oxycedrus L. J. phoenicia L., J. foetidissima Willd, J. sabina
L. and J. exelsa M. Bieb) of them. Juniperus species show antimicrobial and biological activities such
as antitumoral, antifertility, abortifacient, diuretic, anti-inflammatory and antinociceptive. Also some
researchs indicate that it has positive effect on inhibiting cancer because of it’s phenolic content. The
aim of this study is to investigate optimum operating conditions of supercritical carbon dioxide
exctraction to maximize amounts of quercetin and apigenin in Juniperus excelsa M. Bieb berries. For
this purpose, extraction process handled in the range of 40-70oC, 130-230 bar, 30-150 min and 3-7
L/min CO2 flow rate with containing %2 ethanol. The amounts of extracted quercetin and apigenin
were identified by high performance liquid chromatography (HPLC). Optimum operating conditions
were examined by using Design Expert 12 program for each substance via response surface
methodology (RSM). The RSM predicted that the maximum extraction amounts of quercetin and
apigenin were achieved respectively as 1,78 μg/g and 1,10 μg/g at the optimal conditions to be 55oC,
230 bar, 7 L/min CO2 and 114 min.