Akademik Veri Yönetim Sistemi
Hacettepe Journal of Biology and Chemistry, cilt.51, sa.1, ss.103-113, 2023 (Hakemli Dergi)
Helicobacter pylori (H. pylori) infection is accepted as the most important chronic bacterial infection. In recent years, it is reported
that the bacteria are developing resistance against the applied antibiotics. In order to increase the success rate, decrease
recurrence and achieve eradication, it is very important to investigate nontoxic biocompatible herbal resources to be used in
addition to antibiotic therapy. Herbal oils, obtained from plants are being used for various purposes for a long time, particularly
in commercial and scientific fields. Therefore, in our study, we chose various herbal oils (Eucalyptus globulus, Juniperus communis,
Rosmarinus officinalis, Thymus vulgaris) that are known to be effective against gastric and gastrointestinal tract diseases which
do not have adequate investigations over H. pylori in the literature, and we aimed to investigate their antimicrobial activity over
H.pylori and cytotoxic activity on Human Umbilical Vein Endothelial Cells (HUVEC) cell line. The antimicrobial activity is investigated
by microdilution assay (MIC, MBC), and cytotoxic activity is investigated by MTT and LDH assays. As a result, it was found
that Eucalyptus globulus (MIC: 2,81 %v/v, MBC: 5,62 %v/v), Juniperus communis (MIC: 0,35 %v/v, MBC: 0,70 %v/v), Rosmarinus
officinalis (MIC: 2,81 %v/v, MBC: 5,62 %v/v), Thymus vulgaris (MIC: 0,70 %v/v, MBC: 1,40 %v/v) oils were effective against H. pylori.
Besides, it was determined that Thymus vulgaris herbal oil had the highest cytotoxic effect, and Eucalyptus globulus herbal oil had
the lowest cytotoxic effect on the HUVEC cell line.