Akademik Veri Yönetim Sistemi
JOURNAL OF ANALYTICAL CHEMISTRY, cilt.63, sa.5, ss.451-454, 2008 (SCI-Expanded)
A flow-injection UV spectrophotometric method was developed for the determination of ambroxol hydrochloride in tablets. The quantitative determination of ambroxol was performed at 245 nm using distilled water as the carrier solvent. In this study, the flow rate, loop volume, and the number of injections per hour were 15 mL/min, 193 mu L, and 100, respectively. The analytical signal of ambroxol was linear in the concentration range of 40-200 mu g/mL. The detection limit and limit of quantification were found as 11.55 and 38.49 mu g/mL, respectively. The results for the determination of ambroxol in tablets, 29.99 +/- 0.23 mg (mean +/- SD), were in good agreement with the labeled quantities (30 mg/tablet). A relatively high recovery value (100.4%) shows the accuracy of the proposed method. Furthermore, the results obtained were in accordance with those obtained by the HPLC method, which were used as a comparison method for the determination of ambroxol HCl, as far as the Student's t-test and Fisher test results were concerned. It was concluded that the proposed flow-injection UV spectrophotometric method was fast, accurate, precise, and suitable for automation in the determination of ambroxol.