Stobadine inhibits doxorubicin-induced apoptosis through a caspase-9 dependent pathway in P815 mastocytoma cells.

BAĞRIAÇIK E. Ü. , Uslu K., Yurtcu E., Stefek M., Karasu Ç.

Cell biology international, vol.31, no.9, pp.979-84, 2007 (Journal Indexed in SCI Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 9
  • Publication Date: 2007
  • Doi Number: 10.1016/j.cellbi.2007.03.008
  • Title of Journal : Cell biology international
  • Page Numbers: pp.979-84
  • Keywords: doxorubicin, stobadine, apoptosis, caspase activity, antioxidant, pyridoindole, N-acetyl cysteine, PYRIDOINDOLE ANTIOXIDANT STOBADINE, VITAMIN-E, BREAST-CANCER, IN-VIVO, RATS, CARDIOMYOPATHY, CHEMOTHERAPY, DEXRAZOXANE


Doxorubicin (DOXO), a widely used chemotherapeutic agent, induces apoptosis in transformed and non-transformed cells. The apoptotic effect of DOXO has been linked to the generation of reactive oxygen species (ROS). Antioxidants may be effective in the prevention of DOX-induced apoptosis. In the present study we investigated the effects of stobadine, a pyridoindole antioxidant in a DOXO-induced apoptosis model of P815 cells by flow cytometric analyses and by measuring caspase-3 and caspase-9 activities. Pretreating cells with stobadine significantly increased cell viability and decreased apoptosis rate. Inhibition in apoptosis was observed at maximum levels following treatment of cells with 10(-7) M stobadine as evident from flow cytometric analyses. The antiapoptotic effect of stobadine was further confirmed by inhibition of caspase-3 and caspase-9 activities. We found that the antioxidative effects of stobadine were comparable to the effects of a well known antioxidant, N-acetyl L-cysteine (NAC). (C) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.