The aim of this study was to determine the attitudes towards laboratory skills and chemistry laboratory anxieties of pre-service science teachers who were currently registered to general chemistry laboratory course and who had taken this course previously. To evaluate candidates’ attitudes and anxieties in line with this purpose, they were examined in terms of gender, year of study, and the type of high school. The sample of the study consisted of 202 candidates studying in the 1st, 3rd, and 4th years of the Science Education Department of a state university in Ankara. A survey research model was used, and the Attitude Scale towards Laboratory Skills and the Chemistry Laboratory Anxiety Scale were applied. It was determined that candidates’ attitudes were generally scored as “agree” and their anxieties were generally scored as “disagree” in terms of average scores. It was also determined that there was a statistically significant difference in the attitudes and anxieties in terms of gender in favor of males. It was determined that there was no statistically significant difference between attitudes and anxieties in terms of year of study and type of high school. A moderate, negatively significant correlation was found between the average attitude and anxiety scores.