Akademik Veri Yönetim Sistemi
26th ESCV, Frankfurt, Almanya, 18 - 21 Eylül 2024, ss.248, (Özet Bildiri)
AIM: The objective of this study is to assess the effectiveness of using the meningitis/encephalitis(M/E) panel for diagnostic purposes. METHODS: CSF samples from patients with suspected central nervous system (CNS) infection who came to our virology laboratory in January 2023-April 2024 were included in the study. One hundred and fifty two CSF samples were analysed with the multiplex PCR meningitis/encephalitis panel. Demographic data, CSF protein levels, CSF cell counts, blood samples (inflammatory parameters, cell count, serologic test results), radiologic results, and clinical data of the patients with suspicions of CNS infection were collected manually from medical records. RESULTS: At least one pathogen was detected in 9 (6%) of the 152 samples evaluated. The distribution of the detected pathogens was as follows:three HSV-1,one ‘’E.coli’’ K1,two HHV-6,one ‘’Haemophilus influenzae’’,one ‘’Streptococcus agalactiae’’ and CMV combined positive and one CMV positive. Since the clinical, radiological and other laboratory results of three positive patients (2 HHV-6,’’H.influenza’’) were not compatible with the diagnosis of meningoencephalitis, they were evaluated as false positivity. In the others, which were evaluated as true positivity, the same pathogen was detected in singleplex PCR or CSF culture. In addition, when samples of true cases were evaluated with the Modified Reller criteria, it was observed that these cases could be detected without being missed. CONCLUSIONS: Diagnostic stewardship and developing a diagnostic decision rule approach may improve the appropriate use of the ME Panel. Additionally, it shows that cost savings will be achieved by applying modified Reller criteria to patients with a preliminary diagnosis of CNS infection.