Efficient regeneration system from wheat leaf base segments

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HALİLOĞLU K.

Biologia Plantarum, cilt.50, sa.3, ss.326-330, 2006 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 50 Sayı: 3
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1007/s10535-006-0045-x
  • Dergi Adı: Biologia Plantarum
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.326-330
  • Anahtar Kelimeler: Auxins, Cytokinins, Somatic embryos, Triticum aestivum L.
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Gazi Üniversitesi Adresli: Evet

Özet

Efficient plant regeneration system from leaf base segments of wheat (Triticum aestivum L.) was developed. The factors affecting the callus formation and regeneration capacity of leaf segments of two genotypes; Bobwhite and Pavon 76, were investigated. The highest number of somatic embryos (SE) was obtained on Murashige and Skoog medium supplemented with 2 mg dm-3 2,4-dichlorophenoxyacetic acid + 1 mg dm-3 naphthalenacetic acid (14.7 SE per segment). Highest frequency of embryogenic callus (96 %) and somatic embryo formation (24.3 SE per segment) were achieved in the first segments. The highest plantlet regeneration was obtained after transfer of embryogenic calli to regeneration medium supplemented with 1 mg dm-3 kinetin (6.3 plantlets per segment). © 2006 Institute of Experimental Botany, Academy of Sciences of the Czech Republic.