Research Information System
Cereal Research Communications, vol.31, no.3-4, pp.241-248, 2003 (SCI-Expanded)
Two spring wheat (Triticum aestivum L.) genotypes, Bob White and Fielder, and one winter genotype, NE92458, previously identified as being highly embryogenic, were examined for susceptibility to Agrobacterium tumefaciens-mediated transformation. Cultured immature embryos of these genotypes were inoculated and co-cultivated with a disarmed A. tumefaciens strain C58C1 carrying a binary vector pPTN115 coding for the β-glucuronidase gene (GUS), and a selectable marker, the neomycin phosphotransferase II gene (NPT II). Explant genotype influenced transformation success. We found Bob White and Fielder were susceptible to A. tumefaciens infection whereas NE92458 appeared to be resistant. The transformation efficiencies of two spring wheat genotypes, Bob White and Fielder, were 1.0 (2/200) and 0.8% (2/250), respectively. Stable integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis of transformants. Both marker genes, gus and nptII, were expressed and co-segregated in the T1 progeny plants. Three out of four cases, the genes showed Mendelian segregation predicted for transgene insertion at a single locus.