Isolation of amygdalin epimer at high diastereomeric purity and its structural characterization by spectroscopic and Q-TOF LC-MS methods

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Sabir P. R., ÇAPAN İ., AYSAL A. İ., Servi S.

JOURNAL OF CHEMICAL METROLOGY, vol.15, no.2, pp.172-185, 2021 (ESCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 15 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.25135/jcm.60.2105.2076
  • Journal Name: JOURNAL OF CHEMICAL METROLOGY
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.172-185
  • Keywords: Amygdalin, neoamygdalin, cyanogenic glycoside, LC-MS Q-TOF, chromatography, QUANTITATIVE-DETERMINATION, CYANOGENIC GLYCOSIDES, ARMENIACAE SEMEN, RACEMIZATION, PREVENTION, SEPARATION, LAETRILE
  • Gazi University Affiliated: Yes

Abstract

Amygdalin, a cyanogenic glycoside mostly found in the bitter almond kernel, plays an important role in conventional and modern medicine applications. The high diastereomeric purity amygdalin was isolated with chromatographic purification methods and recrystallization. Amygdalin and neoamygdalin were separated chromatographically using the porous graphitic carbon column. Identification and confirmation of each molecule was performed with the ESI Quadrupole Time of Flight system coupled with a reversed phase LC.