Akademik Veri Yönetim Sistemi
BANGLADESH JOURNAL OF BOTANY, cilt.45, sa.1, ss.55-61, 2016 (SCI-Expanded)
Anthemis pestalozzae Boiss. is rare endemic plant of Southern Eastern Turkey. Biotechnological approaches are desired as alternative for ex situ conservation. This study aimed at evaluating factors affecting seed dormancy release and micropropagation of A. pestolozzae. The seed dormancy of A. pestalozzae was released with 2 mg/l GA(3) treatment under dark conditions. Cotyledonary leaf and leaf explants pretreated with 50 mg/l BAP for 72 hrs were maintained on MS medium supplemented with different combinations of BAP and NAA or without any hormone. The maximum number of (12.42) shoots were induced on leaf explants using MS medium containing 1 mg/l BAP + 0.5 mg/l NAA. Whereas, maximum number of shoots on cotyledonary leaf explants were 5.00 on MS medium containing 1.5 mg/l BAP + 0.75 mg/l NAA. Well developed shoots were rooted on half strength of MS medium containing 0.5 mg/l IBA. The rooted and acclimatized plants flowered under greenhouse conditions.