Akademik Veri Yönetim Sistemi
Turkish Journal of Botany, cilt.43, sa.1, ss.27-37, 2019 (SCI-Expanded)
Aluminum (Al) toxicity is a serious factor restricting crop productivity in acid soil, and Al is the major cause of phytotoxicity.However, the role of Al toxicity in interprimer binding site (iPBS) polymorphism, genomic instability, and DNA methylation has notbeen fully investigated. In the current study, the effects of different Al concentrations on iPBS polymorphism, genomic instability, andDNA methylation were investigated in seedlings of three wheat cultivars: Haymana 79, Kılçıksız, and Bezostaja 1. A higher aluminumconcentration increased the polymorphism rate of the iPBS profile, but decreased genomic template stability in all cultivars. A higherAl concentration was found to cause DNA methylation. Furthermore, the coupled restriction enzyme digestion-iPBS technique wasused to detect DNA cytosine methylation level, which could help in understanding the epigenetic mechanism. The occurrence ofhypermethylation and hypomethylation was observed with respect to Al stress treatment, and Al was found to cause DNA methylation.Polymorphism in the CRED-iPBS profile and DNA methylation can be correlated to evaluate epigenetic changes under stress.